Borrelia Burgdorferi (Lyme) - IgM


The MP Diagnostics test kit is based on the principle of the enzyme immunoassay (EIA). Borrelia burgdorferi antigen is bound on the surface of the microtiter strips.

 Description

 Product Description

Diluted patient serum or ready-to-use standards are pipetted into the wells of the microtiter plate. A binding between the IgM antibodies of the serum and the immobilized Borrelia burgdorferi antigen takes place. After a one hour incubation at room temperature, the plate is rinsed with diluted wash solution, in order to remove unbound material. Then ready-to-use anti-human-IgM peroxidase conjugate is added and incubated for 30 minutes. After a further washing step, the substrate (TMB) solution is pipetted and incubated for 20 minutes, inducing the development of a blue dye in the wells. The color development is terminated by the addition of a stop solution, which changes the color from blue to yellow. The resulting dye is measured spectrophotometrically at the wavelength of 450 nm. The concentration of IgM antibodies is directly proportional to the intensity of the color.

 
Brand

MP Diagnostics

 

Regulatory Compliance

CE marked

 

 

Product Type

Kits & Assays

Species Reactivity

Human

Kit or Assay Type

The MP Diagnostics Borrelia burgdorferi IgG ELISA kit is for the detection and quantitative determination of human IgG antibodies against Borrelia burgdorferi in serum and plasma.

Sample Type

Serum; Plasma

Analyte(s) Detected

Borrelia burgdorferi

Sample Volume

100 uL

Evaluation

Quantitative

Diagnostic Platforms

ELISA

Diagnostic Solutions

Infectious Disease

Disease Screened

Lyme

Ig Detected

IgM

Pathogen

Borrelia burgdorferi

Packsize Numeric Quantity

96 wells

Regulatory Compliance

For Research Use Only (RUO)

Product Type

Kits & Assays

Species Reactivity

Human

 

 

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Product Details

Product Name

Description

Order Code

Borrelia Burgdorferi (lyme)

IgG 07I559002 (96 wells)

IgM 07I559202 (96 wells)