HIV-1/2 ELISA 4.0

The MP Diagnostics HIV-1/2 ELISA 4.0 is an antigen sandwich immunoassay.


Product Overview

The MP Diagnostics HIV-1/2 ELISA 4.0 is an antigen sandwich immunoassay. The wells of the polystyrene microplate strips are coated with recombinant HIV antigens (gp41, gp120, and gp-36) expressed in E.coli. The conjugate is based on a second set of recombinant HIV antigens expressing the same epitopes as the pre-coated antigens, which is conjugated to horseradish peroxidase. Human serum or plasma, are incubated in these coated wells. HIV-1/2 specific antibodies, if present, will bind to the antigens immobilised on the solid phase. After incubation, the wells are thoroughly washed to remove unbound materials and conjugate is added to the wells. The antigens of the conjugate will bind to any antigen-antibody complexes previously formed and excess unbound conjugate are removed by washing. Colourless solutions containing urea peroxide and tetramethylbenzidine are then added to each well. The presence of specific antibodies is indicated by the presence of a blue colour after incubation, which changes to yellow when the colour reaction is terminated by the addition of sulphuric acid. The intensity of the resulting yellow product is measured at 450nm using a spectrophotometer and is proportional to the amount of antibodies present in the specimen.


Product Description

The MP Diagnostics (MPD) HIV-1/2 ELISA 4.0 is an enzyme-linked immunosorbent assay (ELISA) intended for the detection of antibodies to Human Immunodeficiency Viruses (HIV) type 1 (group M - O) or type 2 in human serum or plasma samples.



MP Diagnostics


Regulatory Compliance

CE marked


Product Type

Kits & Assays

Species Reactivity


Kit or Assay Type



Microplate|Reactive Control I & II|Non-reactive Control|Conjugate|Plate Wash Concentrate|Diluent|Chromogen Solution A|Chromogen Solution B|Stop Solution

Sample Type

Serum; Plasma

Analyte(s) Detected

HIV-1 and HIV-2 Antibodies

Sample Volume

100 uL

Detection Method


Storage & Handling

Store at 2 - 8 degrees Celsius



Detection/Incubation Time

30 min||30 min||15 min

Diagnostic Platforms


Diagnostic Solutions

Infectious Disease

Disease Screened


Ig Detected

IgA; IgM; IgG


Human Immunodeficiency Virus (HIV) Types 1 and 2

Packsize Numeric Quantity

96-test kit

Regulatory Compliance

CE marked


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Product Details

Product Name


Order Code

HIV-1/2 ELISA 4.0

0723030096 (96 wells)

0723030480 (96 wells x 5)